Short communication: Establishment of a new polymerase chain reaction–sequence-based typing method for genotyping cattle major histocompatibility complex class II DRB3
نویسندگان
چکیده
منابع مشابه
Precise prediction of major histocompatibility complex class II-peptide interaction based on peptide side chain scanning
We describe here a new method for predicting class II major histocompatibility complex-binding peptides, based on the preferences observed in a systematic series of peptide binding experiments where each position in a "minimal" peptide was replaced individually by every amino acid. The DRB1*0401 peptide binding preferences were determined and incorporated into a computer program that looks thro...
متن کاملGenotyping BoLA-DRB3 alleles in Brazilian Dairy Gir cattle (Bos indicus) by temperature-gradient gel electrophoresis (TGGE) and direct sequencing.
BoLA-DRB3 is a gene of the major histocompatibility complex (MHC) in cattle. The product of the BoLA-DRB3 gene is a beta chain of an MHC class II molecule, a glycoprotein expressed on the surface of antigen-presenting cells (APCs). Responses of CD4+ T lymphocytes to peptides are dependent on the presentation of peptide ligands bound to class II molecules on APCs. Genotyping of the BoLA-DRB3 gen...
متن کاملIi Chain Controls the Transport of Major Histocompatibility Complex Class II Molecules to and from Lysosomes
Major histocompatibility complex class II molecules are synthesized as a nonameric complex consisting of three alpha beta dimers associated with a trimer of invariant (Ii) chains. After exiting the TGN, a targeting signal in the Ii chain cytoplasmic domain directs the complex to endosomes where Ii chain is proteolytically processed and removed, allowing class II molecules to bind antigenic pept...
متن کاملA general model of invariant chain association with class II major histocompatibility complex proteins.
The binding of invariant chain to major histocompatibility complex (MHC) proteins is an important step in processing of MHC class II proteins and in antigen presentation. The question of how invariant chain can bind to all MHC class II proteins is central to understanding these processes. We have employed molecular modeling to predict the structure of class II-associated invariant chain peptide...
متن کاملAllelic Diversity of Major Histocompatibility Complex Class II DRB Gene in Indian Cattle and Buffalo
The present study was conducted to study the diversity of MHC-DRB3 alleles in Indian cattle and buffalo breeds. Previously reported BoLA-DRB exon 2 alleles of Indian Zebu cattle, Bos taurus cattle, buffalo, sheep, and goats were analyzed for the identities and divergence among various allele sequences. Comparison of predicted amino acid residues of DRB3 exon 2 alleles with similar alleles from ...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 2009
ISSN: 0022-0302
DOI: 10.3168/jds.2008-1999